常用方法步骤如下：

 

Common methods are as follows:

 

1．洁净厂房设计施工平板检验法：用常规培养基，倒成平板，收入接种室。在事先熏蒸好的接种室，使用前半小时或1小时把供试的培养皿或试管打开，按预定时间盖好培养皿或试管。留对照皿不打开。然后一起放入30℃的温箱中培养48小时，检查有无菌殖生长，并由菌落形态，判断杂菌种类。一般要求平板培养基开盖5分钟，菌落不超过3个；叙面培养基开塞 30分钟者，以不出现菌落为合格。

 

1 clean workshop design and construction of flat panel test method: using conventional culture medium, pour into plate, income inoculation room. In the first half hour or 1 hours to open the test plate or the test tube to open, cover a good culture dish or a test tube. Leave the control dishes do not open. And then, 48 hours, 30 hours, the culture of sterile growth, and by the colony morphology, determine the type of miscellaneous bacteria. General requirements for plate culture Jikaigai 5 minutes and colony does not exceed 3; Syrian surface medium glycerine 30 minutes, to does not appear to be a colony for qualified.

 

2．洁净厂房设计施工 http://www.jnjkjh.com/ 斜面检验法：将常用的琼脂斜面培养基各取二管， 放入接种室，按无菌操作各将其中的一管的棉塞取出，放在灭菌的培养皿内。经过一定时间后，再将棉塞通过火焰，塞回试管，连同对照一起培养。经48小时后，检验有无杂菌生长。和上边方法同。

 

2 clean workshop design and construction method: the commonly used agar slant medium each take two tube, into the inoculation room, according to the aseptic operation each will be one of the tube of the cotton plug out, placed in the sterile culture dish. After a certain period of time, then the cotton plug through the flame, plug back into the test tube, along with the control to develop. After 48 hours, the growth of the bacteria was tested. And the above method.

 

3．洁净厂房设计施工空气污染情况检验：在接种过程中，人员的出过、器材的搬放、接种操作的动作等原因造成空气污染，检验方法是在准备工作结束后，接种操作开始时，按上述方法打开培养皿盖子或试管塞，经5分钟、30分钟或直到工作结束时再盖好，以检验在不同的使用时间内，空气污染的程度。

 

3 clean plant design and construction of air pollution: in the course of vaccination, personnel, equipment, equipment, operation and other reasons to cause air pollution, test method is in the preparation of the work, after the start of the operation, according to the above method to open the lid or test tube plug, 5 minutes, 30 minutes or until the end of the work, to test the extent of air pollution in different time.

 

如霉菌较多时；可先用5％石炭酸全面喷射后，再用甲醛熏蒸。如细菌较多时，可采取用乳酸和甲醛交替熏蒸的办法加以杀灭。

 

Such as mould more; first with 5% overall after spraying with phenol, formaldehyde fumigation. If the bacteria are more, can take the method of alternating with lactic acid and formaldehyde to kill.